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Electrophoretic migration of PH1704 and gelatin protease assay. (A) 10% SDS-PAGE gel of purified PH1704. Lane 1, molecular markers; lane 2, PH 1704 boiled for 20 min in sample buffer; lane 3, PH1704 in sample buffer without boiling. (B) Gelatin overlay of 8% SDS-PAGE gel of purified PH1704. Proteolytic activity is manifested by cleared zones in the dark background. Molecular weights indicated are based on molecular markers in gelatin-SDS-PAGE gel.
