Fig. 4.
Effects of intracellular GTPγS on the 5-HT2AR-induced inward current and increase in sEPSCs. (A) Cell pairs were patched with electrodes filled with intracellular solution containing either GTP or GTPγS. Intracellular perfusion with GTPγS (>10 min) rendered the inward current induced by αm-5-HT (10 μM) effectively irreversible (Upper) but had no detectable effect on the increase in sEPSCs (n = 13 pairs) (Lower). (Inset) Image illustrating the recording configuration used for this experiment. (B) Essentially identical results were observed in cortical slices derived from adult (postnatal day > 33) rats by using 5-HT as an agonist (10 μM, n = 7 control and n = 6 GTPγS-loaded neurons).