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Comparison of intensity change of Trp Raman bands in WT Mb and in Trp mutants of Mb. (A–C) Time-resolved difference spectra of WT Mb, W7F-Mb and W14F-Mb, respectively. The top trace in each panel is the UVRR spectrum of MbCO. Spectra in each panel have been offset for clarity. (D) The changes of integrated intensity of W18 and W16 bands in WT and the two mutant Mbs relative to the intensity in the individual probe-only spectra against a delay time.
