Figure 4.

(A) The proteins asFP595 and asCP562 isolated from A. sulcata and cloned asCP562 exhibit orange fluorescence when they are blotted on a nitrocellulose membrane. (B) A second fluorescent band at twice the molecular mass of asFP595 becomes visible if the protein is denatured without β-mercaptoethanol. This band is not detectable for asCP562 denatured without β-mercaptoethanol. The proteins were excited with broadband UV radiation. Nitrocellulose membranes were photographed by using a 610-nm long-pass filter. (C) All fluorescent proteins on the membrane have an emission maximum at 595 nm.