Table 1.
Protein name | Determined molecular mass, kDa | Calculated molecular mass, kDa | Number of amino acids | Absorption/excitation maxima, nm | Emission maxima, nm |
---|---|---|---|---|---|
asFP499/522 | 26.2*/23.0†/66.0‡ | — | — | <280/≈400/480/511 | 499/522 |
asFP499 (cloned) | 26.2* | 25.4 | 228 | 278/403/480 | 499 |
asFP595 | 19.1*/66.0†‡ | — | — | 278/337/574 | 595 |
asCP562 | 19.1*/66.0†‡ | — | — | 562 | 595§ |
asCP562 (cloned) | 19.1*/66.0†‡ | 16.5 | 148 | 562 | 595§ |
Determined by SDS/PAGE.
Determined by gel filtration under denaturing conditions.
Determined by gel filtration under physiological conditions.
After a denaturation-renaturation process.