Table 2.
Treatment | 65°C/5 min | 80°C/5 min | 95°C/2.5 min | 1% SDS | 8 M Urea | pH 5.5 | pH 11 | 200 mM β-Mercaptoethanol | Mineral oil | 4% Paraformaldehyde |
---|---|---|---|---|---|---|---|---|---|---|
asFP499/522* | +++++ | + | + | +++ (502 nm)† | +++++ | +++ | ++ (509 nm)† | +++++ | ++++ | (d.) |
asFP595* | +++++ | ++++ | ++ | ++ | ++++ (600 nm)† | +++ | + | +++++ | +++++ | (d.) |
asCP562 | (d.) | (d.) | (d.) | (d.) | (d.) | (d.) | (n.d.) | (d.) | (d.) | (d.) |
(d.), Color detectable; (n.d.), color not detectable.
Values are given as % of the peak maximum as compared to untreated samples: +++++ = >90%; ++++ = 75–79%; +++ = 30–35%; ++ = 15–20%; + = <10%.
Shifts of emission maximum caused by the treatment, points of reading.