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. 2007 May 29;104(23):9691–9696. doi: 10.1073/pnas.0611379104

Fig. 3.

Fig. 3.

Histology of wild-type and hot-homozygous mutant larvae. (A–E) Sections of 20-dph larvae. The gonads are encircled by a yellow line. (A) Wild-type XX. (B) Wild-type XY. (C) XX hot/hot. (D) An XX hot/hot, showing hyperplasic and undifferentiated gonad. (E) An XY hot/hot gonad showing exclusively testicular development. (Scale bars, 0.1 mm.) (A′–E′) Enlargement of the gonadal area in A–E. (Scale bars, 0.05 mm.) In XY hot testis (E′, showing enlargement of the boxed area in E), the germ cells have prematurely initiated meiosis at this larval stage. The area encircled by a black line indicates a cluster of spermatid-like cells. sg, spermatogonia-like cells. Hematoxylin-eosin staining is shown in A–D, and neutral red staining is shown in E.