Figure 3.
Gel electrophoresis of the purified recombinant toxins. (A) Two micrograms of purified recombinant toxin were run on a 12.5% SDS-polyacrylamide gel to show the purity of the preparations. (B) 2 μg of purified recombinant toxin were run on an isoelectric focusing gel (5.5% PAA, pH 5–8). The isoelectric point of rSMEZ-2, rSPE-G, and rSPE-H is similar and was estimated at pH 7–8. The IEP of rSMEZ was estimated at pH 6–6.5.