Table I.
Potency of Recombinant Toxins on Human and Mouse T Cells
Proliferation potential P50 [pg/ml] | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Toxin | Human | SJL | B10.M | B10/J | C3H | BALB/c | ||||||
SEA | 0.1 | 20 | 12 | 1.8 | 19 | 1,000 | ||||||
SEE | 0.2 | 10 | 12 | 1.5 | 50 | 15 | ||||||
SEB | 0.8 | 7,000 | 80,000 | 5,000 | 10,000 | 1,000 | ||||||
TSST | 0.2 | 20 | 1,000 | 1.2 | 100 | 10 | ||||||
SPE-C | 0.1 | >100,000 | >100,000 | >100,000 | >100,000 | >100,000 | ||||||
SMEZ | 0.08 | 80 | 80 | 100 | 9,000 | 200 | ||||||
SMEZ-2 | 0.02 | 100 | 15 | 10 | 800 | 18 | ||||||
SPE-G | 2 | >100,000 | >100,000 | >100,000 | >100,000 | >100,000 | ||||||
SPE-H | 50 | 15 | 800 | 5,000 | 100 | 1,000 |
Human PBLs and mouse T cells were stimulated with varying amounts of recombinant toxin. The P50 value reflects the concentration of recombinant toxin required to induce 50% maximal cell proliferaton. No proliferation was detected for rSPE-C and rSPE-G at any concentration tested on murine T cells.