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. 1999 Jan 4;189(1):169–178. doi: 10.1084/jem.189.1.169

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Lymph node and spleen cells from A18 TCRtg mice vaccinated with the 5.4 construct, (thick lines), the control vector (thin lines), or untreated (shaded histograms) were analyzed (A) 3 d and (B) 15 d later by three-color FACS^® analysis. The figure shows expression of CD44, CD62-L and CD69 (and BrdU labeling for day 3 after vaccination) on gated CD4 and Vβ8.3-positive T cells.