Table I.
Frequency of C5-specific T Cells per 106 Spleen Cells
| Weeks after vaccination | Untreated | DNA vaccinated | A18TCRtg untreated | |||
|---|---|---|---|---|---|---|
| 0 | 5 | 5 | 10,000 | |||
| 4 | 5.7 | 110 | 13,333 | |||
| 8 | 5 | 66.6 | 10,000 | |||
| 12 | 5 | 50 | 8,000 | |||
| 16 | 5.7 | 30 | 13,333 | |||
| 20 | 5 | 25 | 10,000 | |||
| 24 | 5.7 | 25 | 10,000 | |||
| 28 | 5 | 25 | 8,000 | |||
| 32 | 5 | 25 | 10,000 | |||
| 36 | 5 | 25 | 13,333 | |||
| 40 | 5 | 25 | 10,000 |
The frequency of C5-specific T cells per 106 spleen cells was calculated by regression analysis of the number of negative wells (24 replicates per cell concentration) at each dilution of responder cells.