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Decreased α-CD3–mediated deletion of DP thymocytes in FTOCs from mIκB-α transgenic mice. Thymi from embryonic day 17.5 wild-type (WT) or mIκB-α transgenic (Tg) mice were cultured for 72 h on semipermeable membranes in DMEM-10 plus 10% FCS in the absence (untreated) or presence of 10 μg/ml α-CD3 mAb (α-CD3). Thymocytes were analyzed by flow cytometry with PE–α-CD4 and FITC– α-CD8. Percentages of cells in the individual subpopulations are shown in the lower left quadrant.
