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. 2000 Jul 3;192(1):11–22. doi: 10.1084/jem.192.1.11

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Interaction of K1 with μ chains of BCR. (A) Interaction of K1 protein with μ chains in BJAB cells. BJAB cells were transfected with pDEF3 vector or pDEF3 vector containing the flag-tagged K1. Cell lysates were used for precipitation (IP) with an anti-flag antibody, followed by an immunoblot (IB) with an anti-μ antibody. Whole cell lysates (WCL) of transfected cells were included as controls. Arrow indicates the μ chain. (B) Interaction of K1 protein with μ chains in the absence of other BCR components. 293 cells were transfected with expression vector containing the flag-tagged K1 and/or expression vector containing human μ chain cDNA as indicated at the bottom of the figure. Cell lysates were used for precipitation (IP) with an anti-flag antibody or anti-μ antibody, followed by an immunoblot (IB) with anti-flag and anti-μ chain antibodies. Whole cell lysates (WCL) of transfected cells were included as controls. Arrow indicates the μ chain and the flag-tagged K1, and the asterisk indicates the heavy chain of antibody. All data were reproduced in at least two independent experiments.