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. 2000 Jul 3;192(1):11–22. doi: 10.1084/jem.192.1.11

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Interaction of the NH₂-terminal region of K1 with μ chains. BJAB cells were transfected with expression vector containing GST or K1/GST. Cell lysates were used for pull-down assay with glutathione beads, followed by an immunoblot (IB) with an anti-μ chain antibody (left). Whole cell lysates (WCL) of transfected cells were included as controls. The expression level of GST-AU1 and K1/GST-AU1 in GST pull-down complexes was demonstrated by immunoblot with an anti-AU1 antibody (right). Arrows indicate the μ chain, GST-AU1, and K1/GST-AU1 protein. The data were reproduced in two independent experiments.