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. 2000 Jul 3;192(1):11–22. doi: 10.1084/jem.192.1.11

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© 2000 The Rockefeller University Press

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graphic file with name JEM000328.f3a.jpg — The NH₂-terminal region of K1 is required for downregulation of BCR surface expression. (A) Summary of BCR downregulation by K1/CD8 chimeras. K1 and CD8α are indicated by hatched and open boxes, respectively. Ex, extracellular region; Tm, transmembrane; Cyt, cytoplasmic region. (B) IgM surface expression. Live BJAB, BJAB/K1, BJAB/K1/CD8-Ex+Tm, BJAB/K1/CD8-Ex, BJAB/K1/CD8-Tm, and BJAB/CD8-Ex+Tm cells were stained for the surface expression of IgM as described in Materials and Methods. As a control, a histogram of each cell line (shaded) is overlaid with a dotted-line histogram of an anti–mouse total IgG antibody as isotype control. The mean value of the relative level of IgM surface expression is presented inside of the figure. The data were reproduced in three independent experiments. Ex, extracellular region; Tm, transmembrane.

graphic file with name JEM000328.f3b.jpg — The NH₂-terminal region of K1 is required for downregulation of BCR surface expression. (A) Summary of BCR downregulation by K1/CD8 chimeras. K1 and CD8α are indicated by hatched and open boxes, respectively. Ex, extracellular region; Tm, transmembrane; Cyt, cytoplasmic region. (B) IgM surface expression. Live BJAB, BJAB/K1, BJAB/K1/CD8-Ex+Tm, BJAB/K1/CD8-Ex, BJAB/K1/CD8-Tm, and BJAB/CD8-Ex+Tm cells were stained for the surface expression of IgM as described in Materials and Methods. As a control, a histogram of each cell line (shaded) is overlaid with a dotted-line histogram of an anti–mouse total IgG antibody as isotype control. The mean value of the relative level of IgM surface expression is presented inside of the figure. The data were reproduced in three independent experiments. Ex, extracellular region; Tm, transmembrane.