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. 2000 Jul 3;192(1):11–22. doi: 10.1084/jem.192.1.11

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localization of IgM. BJAB/babe (BJAB) cells and BJAB/K1 cells were fixed and colabeled with anti-μ antibody (αμ) together with anticalnexin (αcalnexin) or anti–Golgi compartment 58K (αGolgi 58K) antibody. The μ chain was detected with a secondary antibody conjugated with Alexa 568 (red). Calnexin for ER localization and 58K for Golgi compartment localization were detected with a secondary antibody conjugated with Alexa 488 (green). Yellow color at merged images indicates colocalization of the red and green labels. The data were reproduced in two independent experiments.