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. 2000 Jul 3;192(1):11–22. doi: 10.1084/jem.192.1.11

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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K1 localization. Expression vector containing (A) the flag-K1 or (B) the K1-myc was electroporated into BJAB cells. After 48 h, cells were fixed and reacted with mouse monoclonal anti-flag antibody (A) or anti-myc antibody (B). K1 protein was detected with anti–mouse secondary antibody conjugated with Alexa 568 (red). The ER and Golgi compartment were detected with Alexa 488–conjugated Con A (green) and BODIPY C5FL-ceramide (green), respectively. Localization of fluorescently labeled antibodies was visualized with a Leica TCS-SP laser scanning microscope. Yellow color indicates colocalization of the red and green labels. The data were reproduced in two independent experiments.