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. 2000 Jul 3;192(1):117–122. doi: 10.1084/jem.192.1.117

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© 2000 The Rockefeller University Press

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Kinetics of expansion and contraction of activated CD4 T cells during BCG infection. (A) Typical FACS^® histogram plots of CD44 expression on live CD4 T cells of uninfected mice or BCG-infected wild-type and IFN-γ KO mice at 4.5 wk of BCG infection. The CD44 marker was always set on uninfected mice as shown. The CD4 T cell population of IFN-γ KO mice is extremely skewed toward high levels of expression of CD44. (B) Total number of CD4⁺CD44^hi T cells in the spleens of wild-type and IFN-γ KO mice during BCG infection. Cells were stained and analyzed by flow cytometry to measure CD4 and CD44 expression. Shown is the total number of CD4⁺CD44^hi T cells, calculated as: percentage of live cells that are CD4⁺CD44^hi × total number of live splenocytes. Numbers shown are average and SD of six to eight mice per time point, done in two to three experiments per time point. (C) Kinetics of expression of high levels of CD44 on splenic CD4 T cells during BCG infection. Numbers shown are percentage of live CD4 T cells with CD44^hi. This is the average and SD of six to eight mice per group, done in replicate experiments.