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. 2000 Jul 3;192(1):63–76. doi: 10.1084/jem.192.1.63

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Intracellular staining for IFN-γ, MIP-1β, and TNF-α in HIV-specific or CMV-specific CD8⁺ T cells. PBMCs from HIV-infected patients were incubated for 6 h with brefeldin A in the presence of PBS (no activation) or specific peptides (10 μM) (activation). Intracellular staining for IFN-γ, MIP-1β, and TNF-α was carried out, and the cells were analyzed by flow cytometry. Cytokine staining is shown on CMV (A) and HIV (B) specific CD8⁺ T cell populations gated using the tetramers (top). Percentages of cells present in quadrants are shown. Representative data are shown (see Table ).