Table 2.
Cytokine Expression in Antigen-specific CD8 T Cells
| Percentage of positive cells | ||||
|---|---|---|---|---|
| Donor | Specificity | IFN-γ positive | MIP-1β positive | TNF-α positive |
| H1 | A2 gag | 72 | ND | 48 |
| H2 | A2 gag | 66 | ND | 29 |
| H3 | A2 gag | 86 | 75 | 47 |
| H4 | A2 gag | 86 | 85 | 25 |
| H5 | A2 gag | 98 | 86 | 90 |
| H6 | A2 gag | 80 | 80 | 80 |
| H7 | B8 gag | 88 | 80 | 71 |
| H8 | B8 nef | 78 | ND | ND |
| H9 | B8 nef | 25 | 40 | 20 |
| H10 | B8 nef | 80 | ND | ND |
| H11 | B8 nef | 60 | ND | 45 |
| H12 | B8 p24 | 60 | ND | 45 |
| H13 | B27 gag | 47 | 54 | 25 |
| H14 | B27 gag | 24 | 21 | 14 |
| H15 | B57 gag | 68 | 70 | 37 |
| C1 | A2 cmv | 66 | 62 | 49 |
| C2 | A2 cmv | 74 | 73 | 42 |
| C3 | A2 cmv | 55 | 53 | 39 |
| C4 | A2 cmv | 65 | 64 | 50 |
| C5 | A2 cmv | 83 | 83 | 60 |
| C6 | A2 cmv | 95 | 94 | 90 |
| C7 | B7 cmv | 95 | 95 | 95 |
| C8 | B7 cmv | 88 | 85 | 90 |
| C9 | B7 cmv | 76 | 73 | 56 |
| C10 | B7 cmv | 74 | 70 | 46 |
| C11 | B7 cmv | 88 | 80 | 60 |
Intracellular staining for IFN-γ, MIP-1β, and TNF-α was carried out in combination with tetramer staining using samples from HIV-infected patients. The table shows the cytokine expression in different antigen-specific CD8+ T cell populations stimulated with specific peptides.