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. 2000 Jul 3;192(1):105–116. doi: 10.1084/jem.192.1.105

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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GATA-3 and c-maf differentially regulate mouse IL-4 and IL-5 promoter activities. EL-4 cells were cotransfected with 1 μg of reporter construct, pIL-4(−766)Luc 13 or pmIL5Luc(1.2) 42 and the transactivators in the expression vector, either pMEGATA3 25 (0, 1, and 2 μg) or pMEc-maf (0, 1, and 2 μg), by DEAE dextran procedure as described previously 20. Transfection efficiency was monitored using 0.1 μg of pRSV-LacZ. pMEc-maf encoding full-length murine c-maf 40 was cloned by PCR. After 36-h incubation, cells were either unstimulated (open bars) or stimulated (filled bars) for 12 h with PMA (10 ng/ml)/ionomycin (1 μM), and luciferase activity was measured in whole cell extracts and normalized to protein mass as described 65. The data is representative of more than three independent experiments.