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. 2000 Jul 3;192(1):105–116. doi: 10.1084/jem.192.1.105

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© 2000 The Rockefeller University Press

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graphic file with name JEM992252.f6a.jpg — GATA-3 induces chromatin remodeling of the IL-4 locus in a Th1 clone, HDK1. Nuclei from PMA/ionomycin/cAMP-stimulated cells were treated with 0 μg/ml (lanes 1, 4, and 7), 9 μg/ml (lanes 2, 5, and 8), and 12 μg/ml (lanes 3, 6, and 9) of DNaseI and probed with a HindIII–PstI fragment (−797 to −306) of the IL-4 gene 41. Arrowheads indicate positions of BamHI cleavage sites. Locations of the HS sites and a probe are indicated by arrows and a thick bar, respectively.

graphic file with name JEM992252.f6b.jpg — GATA-3 induces chromatin remodeling of the IL-4 locus in a Th1 clone, HDK1. Nuclei from PMA/ionomycin/cAMP-stimulated cells were treated with 0 μg/ml (lanes 1, 4, and 7), 9 μg/ml (lanes 2, 5, and 8), and 12 μg/ml (lanes 3, 6, and 9) of DNaseI and probed with a HindIII–PstI fragment (−797 to −306) of the IL-4 gene 41. Arrowheads indicate positions of BamHI cleavage sites. Locations of the HS sites and a probe are indicated by arrows and a thick bar, respectively.