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. 2000 Jul 3;192(1):31–40. doi: 10.1084/jem.192.1.31

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Recipients of H2-DMα^−/− grafts are efficient producers of alloantibodies. The presence and level of serum alloantibodies was determined by FACS^®. (A) All three recipient groups produce nearly equal levels of alloantibodies directed against allogeneic class I 7 d after transplant. (B) Serum collected at the time of rejection from recipients of I-A_β ^b−^/− or H2-DMα^−/− grafts, ∼20 d, have an elevated level of class I–specific alloantibodies. (C) Recipient sera did not stain B cells from mice lacking MHC expression (β2m^−/− × I-A_β ^b−^/−). In B and C, data are means ± SD of serum samples from at least three animals.