Table 1.
Cytokine RNA Expression of Donor Grafts
| Donor | |||
|---|---|---|---|
| Cytokine | B6 | H2-DMα2/− | I-Ab−/− |
| IFN-γ | + | + | + |
| IL-15 | +/− | +/− | +/− |
| IL-6 | +/− | +/− | +/− |
| IL-10 | +/− | +/− | +/− |
| MIF | +++ | +++ | +++ |
| LTβ | + | ++ | + |
| TGF-β | ++ | ++ | ++ |
| TNF-α | + | + | + |
| TNF-β | +/− | +/− | +/− |
| IL-2 | − | − | − |
| IL-4 | − | − | − |
| IL-5 | − | − | − |
| IL-9 | − | − | − |
| IL-13 | − | − | − |
| IFN-β | − | − | − |
Cytokine RNA levels were determined by RNase protection analysis of donor tissue harvested 7 d after graft or at the time of rejection. Both type 1 and type 2 cytokines were elevated to an equal extent in each recipient group (shown in Fig. 4). MIF, macrophage migration inhibitory factor; LTβ, lymphotoxin β.