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. 2000 Jul 3;192(1):41–52. doi: 10.1084/jem.192.1.41

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Analysis of pCMV-TGF-β1 DNA and mRNA in tissues by PCR and RT-PCR, respectively, at 5, 14, and 28 d after intranasal administration of pCMV-TGF-β1 and TNBS treatment. RT-PCR detects spliced mRNA message that differs in size from DNA and is thus not due to plasmid DNA contamination of endogenous TGF-β1 mRNA (described in text). Lane M, marker; lanes 1, 4, and 7, lung; lanes 2, 5, and 8, colon; lanes 3, 6, and 9, spleen; lanes 1–3, day 5; lanes 4–6, day 14; lanes 7–9, day 28. In TGF-β1 mRNA, a faint band of 726 bp was also detected because of the presence of unspliced message. One experiment representative of three independent experiments is shown.