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. 2000 Jul 3;192(1):41–52. doi: 10.1084/jem.192.1.41

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Intranasal administration of pCMV-TGF-β1 to mice with TNBS colitis decreases the expression of IL-12Rβ2 chain expression on LP CD4⁺ T cells. Mice were administered TNBS on day 0 with or without intranasal administration of pCMV-TGF-β1. LPMCs were isolated at day 5 and stained with FITC–anti-CD4, streptavidin-PE, and IL-12β2R mAb (described in Materials and Methods). The flow cytometric profile of IL-12β2R expresssion after gating CD4⁺ T cell population is shown. One experiment representative of three independent experiments is shown.