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. 2004 Jan 5;199(1):35–46. doi: 10.1084/jem.20030204

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — Transient expression of CEACAMs in 293 cells allows interaction with Opa_CEA gonococci. (A) 293 cells were transfected with CEACAM1, CEACAM3, CEACAM6, or the empty expression vector (pcDNA). After 2 d, cells were infected with Opa_CEA gonococci. At the indicated time points, the number of internalized bacteria was determined by gentamicin protection assays. The graph shows mean values ± SDs of three independent experiments done in triplicate. (B) Transfected 293 cells were infected with nonopaque (Opa⁻), nonopaque, piliated (Opa⁻/P⁺), or Opa_CEA-expressing (Opa_CEA) gonococci for 1 h and analyzed in gentamicin protection assays. The graph shows mean values ± SDs of two independent experiments done in triplicate.