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. 2004 Jan 5;199(1):35–46. doi: 10.1084/jem.20030204

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Copyright © 2004, The Rockefeller University Press

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Figure 6. — Src kinases connect CEACAM3 engagement with Rac stimulation. (A) 293 cells were transfected with the control vector (pcDNA), CEACAM3, or cotransfected with a kinase-inactive mutant of c-Src (c-Src K297M), infected with Opa_CEA gonococci, and employed in gentamicin protection assays. The graph shows mean values ± SDs of two independent experiments done in triplicate. Whole cell lysates (WCL) of the samples were analyzed for Src expression (bottom). (B) Lysates of cells as in A were prepared before and after infection with Opa_CEA gonococci. GST-CRIB pull-down assays (top) or whole cell lysates (WCL; bottom) of the samples were analyzed with monoclonal anti-Rac antibody.