Table 3.

Electrophysiological Properties of S3 Segment Asn Mutant Channels

Construct	Vo	z	ΔG o	ΔΔG o	t o	τd
	mV		kcal/mol	kcal/mol	ms	ms
WT	−19 ± 3	2.6 ± 0.3	−1.2 ± 0.3	—	4.5 ± 0.3	1.1 ± 0.2
I320N	−1 ± 1	2.7 ± 0.2	−0.1 ± 0.0	1.1	21.4 ± 0.8	2.1 ± 0.2
I321N	−15 ± 4	2.0 ± 0.2	−0.7 ± 0.2	0.5	5.6 ± 0.6	2.3 ± 0.2
Y323N	36 ± 3	1.3 ± 0.1	1.1 ± 0.2	2.3	5.6 ± 0.3	0.9 ± 0.2
F324N	8 ± 2	2.0 ± 0.1	0.4 ± 0.1	1.6	59.6 ± 1.9	4.1 ± 0.3

Parameters are as described in Table .