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. 1985 Aug;120(2):316–322.

Bone marrow transplantation in the rat. III. Structure of the liver inflammatory lesion in acute graft-versus-host disease.

D Leszczynski, R Renkonen, P Häyry
PMCID: PMC1887820  PMID: 3895973

Abstract

The liver is a major parenchymal target organ of acute graft-versus-host disease (aGVHD) after bone marrow transplantation in the rat. The authors have analyzed the nature of cellular infiltrates in the liver using monoclonal antibodies against white cell subsets and investigated the anatomic distribution of the inflammatory cell subsets inside the liver parenchyma. Several types of white cells are present in a normal control liver: In the portal area the T-helper (Th) cells predominate, (surface) immunoglobulin-expressing B cells are present in ample numbers, and most of the phagocytes are Ia-positive. In the central vein area the T-suppressor/killer cells (Tsk) dominate, no B cells are present, and most of the phagocytes are Ia-negative. During aGVHD the number of T cells increases rapidly in the portal area; and after an initial strong increase, the Th/Tsk ratio decreases but remains still above 1. In the central vein area there is also an increase in the number of T cells, compared with that in the syngeneic recipient, but the Th/Tsk ratio rapidly decreases and remains uniformly below 1. During aGVHD the B cells entirely disappear from the portal area, whereas a small but distinct number of mature plasma cells with intracellular immunoglobulin appear in the central vein area. Following irradiation the Ia-positive phagocytic cells entirely disappear from the portal area and decrease distinctly in number in the central vein area. During aGVHD the number of Ia-positive phagocytes increases again in both locations. In the central vein area the positive phagocytes are seen over the background level, and, concomitantly, the Ia-negative phagocytes disappear.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson K., Hansen J. A., Storb R., Goehle S., Goldstein G., Thomas E. D. T-cell subpopulations identified by monoclonal antibodies after human marrow transplantation. I. Helper-inducer and cytotoxic-suppressor subsets. Blood. 1982 Jun;59(6):1292–1298. [PubMed] [Google Scholar]
  2. Barclay A. N. The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593–600. [PMC free article] [PubMed] [Google Scholar]
  3. Beschorner W. E., Pino J., Boitnott J. K., Tutschka P. J., Santos G. W. Pathology of the liver with bone marrow transplantation. Effects of busulfan, carmustine, acute graft-versus-host disease, and cytomegalovirus infection. Am J Pathol. 1980 May;99(2):369–386. [PMC free article] [PubMed] [Google Scholar]
  4. Beschorner W. E., Tutschka P. J., Santos G. W. Sequential morphology of graft-versus-host disease in the rat radiation chimera. Clin Immunol Immunopathol. 1982 Feb;22(2):203–224. doi: 10.1016/0090-1229(82)90038-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ekblom M., Ruutu T., Volin L., Leskinen R., Renkonen R., Häyry P. Immunological monitoring of bone marrow transplant recipients. I. Major leucocyte subclasses in the blood. Scand J Haematol. 1984 Aug;33(2):113–122. doi: 10.1111/j.1600-0609.1984.tb02385.x. [DOI] [PubMed] [Google Scholar]
  6. Fox R., McMillan R., Spruce W., Tani P., Mason D. Analysis of T lymphocytes after bone marrow transplantation using monoclonal antibodies. Blood. 1982 Sep;60(3):578–582. [PubMed] [Google Scholar]
  7. Gratama J. W., Naipal A., Oljans P., Zwaan F. E., Verdonck L. F., de Witte T., Vossen J. M., Bolhuis R. L., de Gast G. C., Jansen J. T lymphocyte repopulation and differentiation after bone marrow transplantation. Early shifts in the ratio between T4+ and T8+ T lymphocytes correlate with the occurrence of acute graft-versus-host disease. Blood. 1984 Jun;63(6):1416–1423. [PubMed] [Google Scholar]
  8. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  9. Köhler G., Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol. 1976 Jul;6(7):511–519. doi: 10.1002/eji.1830060713. [DOI] [PubMed] [Google Scholar]
  10. Lampert I. A., Janossy G., Suitters A. J., Bofill M., Palmer S., Gordon-Smith E., Prentice H. G., Thomas J. A. Immunological analysis of the skin in graft versus host disease. Clin Exp Immunol. 1982 Oct;50(1):123–131. [PMC free article] [PubMed] [Google Scholar]
  11. Leszczynski D., Renkonen R., Häyry P. Localization and turnover rate of rat renal 'dendritic' cells. Scand J Immunol. 1985 Apr;21(4):355–360. doi: 10.1111/j.1365-3083.1985.tb01441.x. [DOI] [PubMed] [Google Scholar]
  12. McMaster W. R., Williams A. F. Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur J Immunol. 1979 Jun;9(6):426–433. doi: 10.1002/eji.1830090603. [DOI] [PubMed] [Google Scholar]
  13. Müller C., Schüch K., Pawelec G., Wilms K., Wernet P. Immunohistology of graft-versus-host disease mediated skin lesions and its correlation to a large granular lymphocyte surface phenotype and function. Blut. 1982 Feb;44(2):89–94. doi: 10.1007/BF00320095. [DOI] [PubMed] [Google Scholar]
  14. Rappeport J. M., Dunn M. J., Parkman R. Immature T lymphocytes in the peripheral blood of bone marrow transplant recipients. Transplantation. 1983 Dec;36(6):674–680. doi: 10.1097/00007890-198336060-00018. [DOI] [PubMed] [Google Scholar]
  15. Reitamo S., Konttinen Y. T., Ranki A., Häyry P. The relation of different inflammatory cell types to the various parenchymal components of rejecting kidney allografts. Histopathology. 1980 Sep;4(5):517–532. doi: 10.1111/j.1365-2559.1980.tb02946.x. [DOI] [PubMed] [Google Scholar]
  16. Renkonen R., Häyry P. Bone marrow transplantation in the rat. I. Histologic correlations and quantitation of cellular infiltrates in acute graft-versus-host disease. Am J Pathol. 1984 Dec;117(3):462–470. [PMC free article] [PubMed] [Google Scholar]
  17. Sale G. E., Storb R., Kolb H. Histopathology of hepatic acute graft-versus-host disease in the dog. A double blind study confirms the specificity of small bile duct lesions. Transplantation. 1978 Aug;26(2):103–106. doi: 10.1097/00007890-197808000-00009. [DOI] [PubMed] [Google Scholar]
  18. Schroff R. W., Gale R. P., Fahey J. L. Regeneration of T cell subpopulations after bone marrow transplantation: cytomegalovirus infection and lymphoid subset imbalance. J Immunol. 1982 Nov;129(5):1926–1930. [PubMed] [Google Scholar]
  19. Seemayer T. A., Gartner J. G., Lapp W. S. The graft-versus-host reaction. Hum Pathol. 1983 Jan;14(1):3–5. doi: 10.1016/s0046-8177(83)80040-9. [DOI] [PubMed] [Google Scholar]
  20. Si L., Whiteside T. L., Van Thiel D. H., Rabin B. S. Lymphocyte subpopulations at the site of "piecemeal" necrosis in end stage chronic liver diseases and rejecting liver allografts in cyclosporine-treated patients. Lab Invest. 1984 Mar;50(3):341–347. [PubMed] [Google Scholar]
  21. Tsoi M. S. Immunological mechanisms of graft-versus-host disease in man. Transplantation. 1982 May;33(5):459–464. [PubMed] [Google Scholar]
  22. Ueda M., Harada M., Shiobara S., Nakao S., Kondo K., Odaka K., Matsue K., Mori T., Hattori K. T lymphocyte reconstitution in long-term survivors after allogeneic and autologous marrow transplantation. Transplantation. 1984 Jun;37(6):552–556. doi: 10.1097/00007890-198406000-00005. [DOI] [PubMed] [Google Scholar]
  23. Williams A. F., Galfrè G., Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977 Nov;12(3):663–673. doi: 10.1016/0092-8674(77)90266-5. [DOI] [PubMed] [Google Scholar]

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