Figure 2.

Cdc6p-dependent protein loading on ARS1 beads. Whole-cell extracts were prepared from YGP82 cells in which Cdc6p was expressed (Cdc6p +) or repressed (Cdc6p −). Wild-type (W) or mutant (M) ARS1 beads were incubated with increasing amounts of the extracts, as indicated by their final concentrations in the reaction mixtures (Extracts). The levels of ORC, Cdc6p, and Mcm proteins associated with the beads (Bead) or in the supernatants (Sup) were detected by immunoblotting as described in Fig. 1.