Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1995 Mar;69(3):1951–1954. doi: 10.1128/jvi.69.3.1951-1954.1995

Capped nonviral sequences at the 5' end of the mRNAs of rice hoja blanca virus RNA4.

B C Ramirez 1, D Garcin 1, L A Calvert 1, D Kolakofsky 1, A L Haenni 1
PMCID: PMC188814  PMID: 7853540

Abstract

Subgenomic RNAs of both polarities corresponding to rice hoja blanca virus (RHBV) ambisense RNA4 were detected in RHBV-infected rice tissues. Total RNA extracted from RHBV-infected and noninfected rice tissues and RNA4 purified from RHBV ribonucleoprotein particles were used as templates for primer extension studies. The RNAs extracted from RHBV-infected tissues contain a population of RNA molecules with 10 to 17 nonviral nucleotides at their 5' end. The RNA-cDNA hybrids resulting from primer extension of such RNA molecules were specifically immunoselected with anti-cap antibodies, indicating that the subgenomic RNAs are capped and probably serve as mRNAs and that the additional nucleotides at their 5' end possibly derive from host mRNAs via a cap-snatching mechanism.

Full Text

The Full Text of this article is available as a PDF (264.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbier P., Takahashi M., Nakamura I., Toriyama S., Ishihama A. Solubilization and promoter analysis of RNA polymerase from rice stripe virus. J Virol. 1992 Oct;66(10):6171–6174. doi: 10.1128/jvi.66.10.6171-6174.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop D. H., Auperin D. D. Arenavirus gene structure and organization. Curr Top Microbiol Immunol. 1987;133:5–17. doi: 10.1007/978-3-642-71683-6_2. [DOI] [PubMed] [Google Scholar]
  3. Bouloy M. Bunyaviridae: genome organization and replication strategies. Adv Virus Res. 1991;40:235–275. doi: 10.1016/s0065-3527(08)60281-x. [DOI] [PubMed] [Google Scholar]
  4. Elliott R. M., Schmaljohn C. S., Collett M. S. Bunyaviridae genome structure and gene expression. Curr Top Microbiol Immunol. 1991;169:91–141. doi: 10.1007/978-3-642-76018-1_4. [DOI] [PubMed] [Google Scholar]
  5. Garcin D., Kolakofsky D. A novel mechanism for the initiation of Tacaribe arenavirus genome replication. J Virol. 1990 Dec;64(12):6196–6203. doi: 10.1128/jvi.64.12.6196-6203.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hacker D., Rochat S., Kolakofsky D. Anti-mRNAs in La Crosse bunyavirus-infected cells. J Virol. 1990 Oct;64(10):5051–5057. doi: 10.1128/jvi.64.10.5051-5057.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huiet L., Feldstein P. A., Tsai J. H., Falk B. W. The maize stripe virus major noncapsid protein messenger RNA transcripts contain heterogeneous leader sequences at their 5' termini. Virology. 1993 Dec;197(2):808–812. doi: 10.1006/viro.1993.1662. [DOI] [PubMed] [Google Scholar]
  8. Jin H., Elliott R. M. Non-viral sequences at the 5' ends of Dugbe nairovirus S mRNAs. J Gen Virol. 1993 Oct;74(Pt 10):2293–2297. doi: 10.1099/0022-1317-74-10-2293. [DOI] [PubMed] [Google Scholar]
  9. Kolakofsky D., Hacker D. Bunyavirus RNA synthesis: genome transcription and replication. Curr Top Microbiol Immunol. 1991;169:143–159. doi: 10.1007/978-3-642-76018-1_5. [DOI] [PubMed] [Google Scholar]
  10. Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R. The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. J Gen Virol. 1992 Nov;73(Pt 11):2795–2804. doi: 10.1099/0022-1317-73-11-2795. [DOI] [PubMed] [Google Scholar]
  11. Kormelink R., van Poelwijk F., Peters D., Goldbach R. Non-viral heterogeneous sequences at the 5' ends of tomato spotted wilt virus mRNAs. J Gen Virol. 1992 Aug;73(Pt 8):2125–2128. doi: 10.1099/0022-1317-73-8-2125. [DOI] [PubMed] [Google Scholar]
  12. Krug R. M. Priming of influenza viral RNA transcription by capped heterologous RNAs. Curr Top Microbiol Immunol. 1981;93:125–149. doi: 10.1007/978-3-642-68123-3_6. [DOI] [PubMed] [Google Scholar]
  13. Law M. D., Speck J., Moyer J. W. The M RNA of impatiens necrotic spot Tospovirus (Bunyaviridae) has an ambisense genomic organization. Virology. 1992 Jun;188(2):732–741. doi: 10.1016/0042-6822(92)90528-w. [DOI] [PubMed] [Google Scholar]
  14. Munns T. W., Liszewski M. K., Tellam J. T., Sims H. F., Rhoads R. E. Antibody-nucleic acid complexes. Immunospecific retention of globin messenger ribonucleic acid with antibodies specific for 7-methylguanosine. Biochemistry. 1982 Jun 8;21(12):2922–2928. doi: 10.1021/bi00541a018. [DOI] [PubMed] [Google Scholar]
  15. Palmer E. L., Obijeski J. F., Webb P. A., Johnson K. M. The circular, segmented nucleocapsid of an arenavirus-Tacaribe virus. J Gen Virol. 1977 Sep;36(3):541–545. doi: 10.1099/0022-1317-36-3-541. [DOI] [PubMed] [Google Scholar]
  16. Raju R., Raju L., Hacker D., Garcin D., Compans R., Kolakofsky D. Nontemplated bases at the 5' ends of Tacaribe virus mRNAs. Virology. 1990 Jan;174(1):53–59. doi: 10.1016/0042-6822(90)90053-t. [DOI] [PubMed] [Google Scholar]
  17. Ramirez B. C., Lozano I., Constantino L. M., Haenni A. L., Calvert L. A. Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4. J Gen Virol. 1993 Nov;74(Pt 11):2463–2468. doi: 10.1099/0022-1317-74-11-2463. [DOI] [PubMed] [Google Scholar]
  18. Ramirez B. C., Macaya G., Calvert L. A., Haenni A. L. Rice hoja blanca virus genome characterization and expression in vitro. J Gen Virol. 1992 Jun;73(Pt 6):1457–1464. doi: 10.1099/0022-1317-73-6-1457. [DOI] [PubMed] [Google Scholar]
  19. Ramírez B. C., Haenni A. L. Molecular biology of tenuiviruses, a remarkable group of plant viruses. J Gen Virol. 1994 Mar;75(Pt 3):467–475. doi: 10.1099/0022-1317-75-3-467. [DOI] [PubMed] [Google Scholar]
  20. Vialat P., Bouloy M. Germiston virus transcriptase requires active 40S ribosomal subunits and utilizes capped cellular RNAs. J Virol. 1992 Feb;66(2):685–693. doi: 10.1128/jvi.66.2.685-693.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. de Haan P., Wagemakers L., Peters D., Goldbach R. The S RNA segment of tomato spotted wilt virus has an ambisense character. J Gen Virol. 1990 May;71(Pt 5):1001–1007. doi: 10.1099/0022-1317-71-5-1001. [DOI] [PubMed] [Google Scholar]
  22. van Poelwijk F., Boye K., Oosterling R., Peters D., Goldbach R. Detection of the L protein of tomato spotted wilt virus. Virology. 1993 Nov;197(1):468–470. doi: 10.1006/viro.1993.1614. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES