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HF extracts from diatom cell walls. Extracts were subjected to Tris-Tricine SDS/PAGE (11) and stained with Coomassie blue. Lanes: 1, C. fusiformis (positions of silaffin species are marked); 2, N. angularis; 3, C. didymum; 4, C. debilis; 5, E. zodiacus; 6, S. turris. The brackets in lane 2 mark the three silaffins that were used for silica precipitation (see Fig. 6).
