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Cdk5 hyperphosphorylates both histone H1 and τ at 3 and 6 h after heat shock. Autoradiographs (A and D) of Coomassie blue-stained (B and E) and immunoblots probed with the Cdk5 antibody (C and F) of SDS/PAGE gels of aliquots of kinase assay mixtures toward histone H1 (A and B) and τ (D and E) show hyperphosphorylation of both histone H1 and τ at 3 and 6 h after heat shock. Note equal amounts of Cdk5, histone H1, and τ in all lanes. C, control.
