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. 2005 Oct;7(4):486–494. doi: 10.1016/S1525-1578(10)60579-X

Figure 1.

Figure 1

Agarose gels of multiplex PCR products stained with ethidium bromide. A: Gel-analyzing PCR products from primary multiplex reactions containing primer pairs for the human rRNA gene as well as all three pathogen primer pairs for detection of B. anthracis, F. tularensis, and Y. pseudotuberculosis (surrogate for Y. pestis). Lane M is the 50-bp GeneRuler (Fermentas) with selected band sizes in bp at left. In lane 1, the template for the reaction was no DNA as a negative control, the remaining lanes contain products from reactions with template DNA from a blood sample spiked with the following amounts of Y. pseudotuberculosis bacteria: lane 2, 5 CFU/ml; lane 3, 50 CFU/ml; lane 4, 500 CFU/ml; lane 5, 5000 CFU/ml; lane 6, blood alone. The arrow indicates the position of the Y. pseudotuberculosis amplicon DNA and the arrowhead indicates the position of the human rRNA amplicon DNA. B: Gel-analyzing PCR products from secondary multiplex reactions each using PCR products from a primary reaction as template, the primer pair for the human rRNA gene and one pathogen primer pair. The lanes are sets of three using the same template in which the first lane has the B. anthracis primer pair, the second lane has the F. tularensis primer pair, and the third has the Y. pestis primer pair. Lanes 1 to 3, template is negative control reaction shown in A, lane 1. Lanes 4 to 6, template is primary PCR reaction with blood alone. Lanes 7 to 9, template is primary PCR reaction with Y. pseudotuberculosis, 5 CFU/ml. Lanes 10 to 12, template is primary PCR reaction with Y. pseudotuberculosis, 50 CFU/ml. Lanes 13 to 15, template is primary PCR reaction with Y. pseudotuberculosis, 500 CFU/ml. Arrows indicate the position of DNA fragments that correspond to the Y. pseudotuberculosis amplicon and the human rRNA amplicon as in A.