Skip to main content
. 2005 Oct;7(4):495–503. doi: 10.1016/S1525-1578(10)60580-6

Figure 3.

Figure 3

Strategy for BIOMED-2 multiplex PCR TCR clonality analysis in suspect T-cell proliferations. In TCRαβ+ or TCR-negative T-cell proliferations, there is no clear evidence-based preference for either the TCRB or TCRG locus as the initial target for clonality diagnostics. Thus, either TCRB or TCRG can be used as the first line target, followed by the other locus as the second target. For TCRγδ+ T-cell proliferations, the TCRG locus is the clear first line target for clonality assessment, followed by TCRD as the second target. In the absence of clonal TCR gene rearrangements in PCR, SB analysis might still be considered for all suspect T-cell proliferations, provided that enough high-quality DNA is available. Although the PCR strategy as described here is suitable for fresh or frozen tissue samples, it could possibly be applied to DNA isolated from paraffin-embedded tissue samples, provided that DNA quality is such that PCR products of ∼300 bp can be amplified.