. 2005 Oct;7(4):504–510. doi: 10.1016/S1525-1578(10)60581-8

Table 1.

Multicolor Real-Time PCR Ehrlichia Genus-Specific dsbPrimers and Homology to Each Species

Ehrlichia species (GenBank accession)	Primer Dsb-321* (forward, 5′– 3′) TTGCAAAATGATGTCTGAAGATATGAAACA T_m(°C) =66.4	Primer Dsb-671^†^‡ (reverse, 5′–3′) GCTGCTCCACCAATAAATGTATCYCCTA T_m(°C) =66.1
E. chaffeensis (AF403711)	… . … … … … … … … … …	. . … … … … … … … . . T… .
E. canis(AF403710)	… . … … … … … … … … …	. . … . . A… . . G… … … . . C… .
E. ewingii(AY428950)	ND	GCAGCTCCACCAATGAATGTATTTCCAA^§

ND, not determined.

The Ehrlichia forward primer sequence corresponds to nucleotides 321 to 350 from dsb in each species.

^†

All Ehrlichia reverse primer sequences correspond to the reverse complement of nucleotides 671 to 698 from dsbin each respective species.

^‡

Y = T or C.

^§

Dsb-671-ew reverse primer is 100% complementary to the E. ewingii dsb sequence.