Figure 1.

AR is modified by SUMO-1. COS-1 cells grown on 6-cm dishes were transfected with vectors encoding Flag-tagged wt AR (wt) or AR lacking most of the DBD and the hinge region (ΔDBD), and GFP-tagged SUMO-1 (SUMO) or SUMO-1GA (SUMOGA) expression vector. The cells received 100 nM testosterone (T) or vehicle 12 h before harvesting as indicated. Five percent of the cell extracts were immunoblotted with AR antibody (A), and the rest were subjected to immunoprecipitation with anti-Flag antibody. The immunoprecipitates were immunoblotted with anti-GFP antibody (B) or anti-SUMO-1 antibody (C). Arrowheads depict the slowly migrating sumoylated forms of AR.