Skip to main content
. 2007 Jun 14;117(7):1968–1978. doi: 10.1172/JCI30060

Figure 2. NPC1L1 localizes to the canalicular membrane in L1-Tg and human liver tissues.

Figure 2

(A) Acetone-fixed frozen sections of liver samples from 3-month-old L1-Tg112 and WT male mice fed the 0.015% cholesterol diet were processed for fluorescence immunohistochemistry with Bsn4052 antibody to human NPC1L1 and mouse monoclonal C219 antibody to ABCB1 as described in Methods. Arrows denote canalicular staining in the section of L1-Tg liver. Original magnification, ×630. Scale bars: 10 μm. (B) Fluorescence immunohistochemistry of human NPC1L1 was conducted as described in Methods, and sections were examined immediately using an Axioplan 2 fluorescence microscope. Images were taken to show the triad region of liver. Green autofluorescence from elastins in the blood vessel wall was included to distinguish bile duct from blood vessels. Arrows denote canalicular pattern (rhodamine red) of human NPC1L1 staining by Bsn4052 in the L1-Tg liver. Green and red stain appears white. a, artery; b, bile duct; v, vein. Original magnification, ×630. (C) Acetone-fixed frozen sections of human liver specimens were processed for fluorescence immunohistochemistry using C219, Bsn4052, and Bsn4052 preimmune serum as described in Methods, and sections were examined using an Axioplan 2 fluorescence microscope. Arrows denote canalicular pattern of human NPC1L1 staining by Bsn4052 and its colocalization with ABCB1 stained by C219. Scale bars: 35 μm.