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. 2007 Jun 14;117(7):1968–1978. doi: 10.1172/JCI30060

Figure 4. Increased plasma cholesterol is mainly carried on the apoE-rich HDL.

Figure 4

(A) Plasma lipoprotein cholesterol distribution in male WT and L1-Tg112 mice was determined as described in Methods. (B) Apolipoprotein distribution of plasma lipoproteins from male WT and L1-Tg112 mice was determined as described in Methods. Data represent a qualitative, not a quantitative, analysis of apolipoproteins. (C) Quantification of plasma apoE levels by immunoblotting. Plasma was collected from 4 WT and 4 L1-Tg112 mice fed the 0.2% cholesterol diet for 21 days. After 1:200 dilution in saline, 14 μl of diluted samples was immunoblotted with a rabbit anti-rat apoE serum and quantified as described in Methods. Values are mean ± SEM of 4 samples. (D) FA composition of lipoprotein CE was determined as described in Methods in male WT and L1-Tg112 mice. Values are the sum of the percentages of saturated (16:0 and 18:0) and monounsaturated (16:1 and 18:1) FAs divided by the percentage of polyunsaturated (18:2, 20:4, 20:5, and 22:6) FAs. *P < 0.05 versus WT (Student’s t test).