Fig. 2.

QRT-PCR validation of microarray-based GEP data. The results for GAD₆₇, HDAC1, LEF1, Runx2, PAX5, and GRIK1/3 show changes in the same direction as those observed with microarray. The error bars are very small; however, the P value of 0.001 is robust for the REST analysis that was used. The error bars for GAD₆₇ appear to be larger than those of the other genes because of the proportions of the graph needed to accommodate the data for all of the genes. On a percent basis, the error bars for GAD₆₇ when expressed as a percent of the mean are relatively similar to those for the other genes.