Table 1.
Subunit composition | Calculated mass/Da | Measured mass/Da | |
---|---|---|---|
MtGimC | MtGimα2MtGimβ4 | 86,848.6 | 86,909.8 ± 17 |
Pentamer | MtGimα2MtGimβ3 | 72,985.9 | 72,999.4 ± 10 |
Tetramer | MtGimα2MtGimβ2 | 59,123.2 | 59,140.4 ± 13 |
Trimer | MtGimα2MtGimβ1 | 45,260.5 | 45,271.6 ± 11 |
MtGimα1MtGimβ2 | 43,424.3 | 43,446.9 ± 14 | |
Dimer | MtGimα2 | 31,397.8 | 31,399.4 ± 2 |
Monomer | MtGimα | 15,698.9 | 15,699.8 ± 1 |
MtGimβ | 13,862.7 | 13,861.9 ± 0.8 |
All masses have been measured from peak series containing at least four charge states. The broad nature of the peaks for the higher oligomers, most likely caused by an association of counterions or water molecules, decreases the accuracy of the m/z values, leading to an increase in the standard deviation (16).