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. 2007 May 10;26(11):2633–2644. doi: 10.1038/sj.emboj.7601706

Figure 4.

Figure 4

Effect of the kinase activity of c-Src on its binding to AF-6. (A) HEK 293 cells were transiently transfected with empty vector (−) or constructs as indicated. The interaction between AF-6 and Src proteins was detected by precipitation with anti-FLAG and blotting with anti-Src antibodies. Protein expression was controlled by blotting with anti-AF-6 and anti-Src antibodies. (B, C) HEK 293 cells were transiently transfected and pretreated with or without the Src kinase inhibitor PP2 1 h before lysis. The interaction between endogenous AF-6 and SrcCT proteins was detected by precipitation with anti-AF-6 and blotting with anti-Src antibodies. Blotting with anti-pY antibody was included for control of Src activity. (D) Endogenous AF-6 was precipitated from Src-L expressing cells and blotted with anti-pY antibody.