Figure 1.

Deletions within the ARF1 SBS indicate that a central core region is required for Stau1 binding in vivo. (A) (Left) Schematic representations of the various 3′-end-deleted mRNAs that derive from pSport-ARF1 SBS derivatives. Numbering is relative to the first nucleotide following the termination codon, which is defined as 1. Plus (+) and minus (−) signs at the right of each representation indicate the ability or failure to bind Stau1, respectively. (Right) Human 293 cells were transfected with a derivative of the pSport-ARF1 SBS test plasmid and a plasmid expressing Stau1-HA₃. After immunopurification (IP) using anti(α)-HA, protein was analyzed using Western blotting (WB) and anti-HA (upper), and RNA was analyzed using RT–PCR and ethidium bromide staining (lower). (B) (Left) as in (A, left) except for the pSport-ARF1 SBS derivatives that were analyzed. (Right) as in A, right. Results are representative of two independently performed experiments.