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. 2007 May 10;26(11):2621–2632. doi: 10.1038/sj.emboj.7601716

Figure 6.

Figure 6

Role of Gβγ and Tctex-1 in neuronal differentiation. (A) Ectopic expression of Gβ1 and Tctex-1 induces multiple, long neurites in hippocampal neurons. Hippocampal neurons were transfected as indicated, with expression vectors for GFP, GFP+FLAG-Gβ1, GFP+FLAG-Tctex-1 and GFP+Tctex-1+βARKct 2 h after plating. The transfected neurons were fixed and processed for GFP fluorescence 24 h after transfection. (B) Expression of Gβγ-sequestering reagent, βARKct inhibits neurite outgrowth. Hippocampal neurons were transfected either 2 h or 12 h after plating, with expression vectors for GFP and βARKct and analyzed 24 h after transfection for GFP expression (green) and Tyr-Tubulin labeling (red). βARKct expression results in arresting the cells in stage 1 when transfected 2 h after plating and in stage 2 when transfected 12 h after plating. Note that, under both conditions, the untransfected cells within the same field appear healthy and have reached stage 3. The images shown here are representative of three independent transfections. Quantification of the images from these transfections is shown in Table I.