Table 1.

Quantitative analyses of morphological changes of transfected neurons

Overexpressed protein	% cells (stage 1)	% cells (stage 2)	% cells with a single neurite (stage 3)	% cells with multiple neurites >70–80 μm
GFP alone	8±4	52±8	40±6	0.6±01
βARKct	78±6*	17±3*	4±2*	ND
FLAG-Gβ1	2±1	36±9*	42±14	22±6*
FLAG-Tctex1	2±1	24±8*	28±6	38±8*
FLAG-Tctex1+βARKct	10±4	38±4*	40±12	12±6*
Cells were transfected at 2 h after plating and fixed 24 h later. Each transfection received 1 μg of GFP expressing vector for visualizing the transfected cells. For all other constructs, 2 μg of plasmid were typically used. The total amount of DNA added was kept constant by adding appropriate amount of control vector. A neurite longer than 70–80 μm was considered to be an axon in these analyses. Each value represents the mean±s.e.m. of at least 50–75 cells for each experimental condition. Asterisk represents value significantly different from that of the GFP-transfected group (P<0.01).
ND, not detected.