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. 2007 May 10;26(11):2607–2620. doi: 10.1038/sj.emboj.7601718

Figure 4.

Figure 4

Prodomain processing is essential for MIG-17 function in cell migration. (A) Processing of MIG-17 in vitro. The lysates from wild-type worms expressing MIG-17-GFP or MIG-17(E303Q)-GFP were incubated at room temperature for the indicated periods and immunoblotted with anti-GFP. (B) Mutant constructs of potential processing sites. (C) The extracts from wild-type animals expressing MIG-17(KK202LL)-GFP were analyzed as in (A). (D) Gonadal localization of MIG-17(KK202LL)-GFP. Scale bar, 20 μm. (E) mig-17 rescue experiments using the constructs in (B). Data for DTC migration are shown as in Figure 1D (n=120). The localization scores are shown on the right (n=20). (F, G) Processing of glycosylation mutants MIG-17(ΔGly7–9)-GFP and MIG-17(ΔGly1–6)-GFP in vitro. Experiments were preformed as in (A). The mature form of MIG-17(ΔGly1–6)-GFP can be detected after a 6-h incubation.