Table 3.

Comparison of results of nested multiplex PCR (Polymerase Chain Reaction) and TechLab E. histolytica II ELISA test performed on stool specimens.

Nested multiplex PCR result	TechLab E. histolytica II stool specimen test resulta		Total no. of specimen
	Positive	Negative
E. dispar (mono infection)	0	8	8
E. histolytica (mono infection)	4	2	6
E. moshkovskii (mono infection)	0	0	0
E. dispar + E. moshkovskii (mixed)	0	2	2
E. dispar + E. histolytica (mixed)	17	1	18
E. histolytica + E. moshkovskii (mixed)	1	0	1
E. dispar+E. histolytica+E. moshkovskii (mixed)	7	3	10
Negative	0	0	0
Total	29	16	45

^a E. histolytica II ELISA test is designed to detect only E. histolytica specific lectin antigen in stool specimen and therefore can confirm only the presence of E. histolytica.