Table 4.

Primer sequences used for nested multiplex PCR

Genus specific primers (First PCR)
Entamoeba genus	E-1 5' TAAGATGCACGAGAGCGAAA 3' (forward primer) E-2 5' GTACAAAGGGCAGGGACGTA 3' (reverse primer)
Species specific primers (Second nested multiplex PCR)
E. histolytica species	EH-1 5' AAGCATTGTTTCTAGATCTGAG 3' (forward primer) EH-2 5' AAGAGGTCTAACCGAAATTAG 3' (reverse primer)
E. moshkovskii species	Mos-1 5' GAAACCAAGAGTTTCACAAC 3' (forward primer) Mos-2 5' CAATATAAGGCTTGGATGAT 3' (reverse primer)
E. dispar species	ED-1 5' TCTAATTTCGATTAGAACTCT 3' (forward primer) ED-2 5' TCCCTACCTATTAGACATAGC 3' (reverse primer)