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Construction of chimeric duplexes. Oligonucleotides used in chimeras and splints are shown. RNA is bold, DNA is in normal lettering, and bulges and ligation junctions are indicated. “p” represents a 5′-phosphate and “OH” a 3′-hydroxyl. (A) Preparation and sequence of DRD chimeras. Shown is base pairing to splints and BS66-R-1. (B) Preparation and sequence of DDR chimeras. Shown is base pairing to splint 1 and BS66-R-2.
